Anti-Chk1 抗体 (ab17191)
Key features and details
- Rabbit polyclonal to Chk1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Chk1 antibody
Chk1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Chk1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human Chk1 aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14757 -
ポジティブ・コントロール
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/1000 - 1/5000.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
|
WB |
1/10000 - 1/25000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
特記事項 |
---|
IHC-P
1/1000 - 1/5000. |
IP
Use at 2-10 µg/mg of lysate. |
WB
1/10000 - 1/25000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
- Information by UniProt
-
参照データベース
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
-
別名
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
画像
-
All lanes : Anti-Chk1 antibody (ab17191) at 0.4 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TMCK (Mouse SV40 transformed kidney cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 54 kDa
Exposure time: 30 seconds -
Detection of Chk1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Lane 1: Anti-Chk1 antibody (ab17191) at 6 µg/mg lysate, Lane 2: Anti-Chk1 antibody (ab 17191) at 6 µg/mg lysate, Lane 3: Control IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 seconds.
-
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Chk1 with ab17191 at 1/1000 dilution. Detection: DAB.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab17191 は論文での使用が確認できていません。