製品の概要

  • 製品名Anti-CHFR antibody
    CHFR 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CHFR
  • アプリケーション適用あり: ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    KFNHICEQTRFKN

    , corresponding to C terminal amino acids 611-623 of Human CHFR.

  • 特記事項GenBank Accession Number - NP_060693

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/64000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • 組織特異性Ubiquitous.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the CHFR family.
    Contains 1 FHA domain.
    Contains 1 PBZ-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 発生段階Weakly expressed in G1 phase, and highly expressed during S phase.
  • ドメインThe PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
    The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.
  • 翻訳後修飾Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Autoubiquitinated; may regulate its cellular level.
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
  • 細胞内局在Nucleus > PML body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Checkpoint with forkhead and ring finger domains antibody
    • Checkpoint with forkhead and RING finger domains protein antibody
    • Chfr antibody
    • CHFR_HUMAN antibody
    • E3 ubiquitin protein ligase CHFR antibody
    • E3 ubiquitin-protein ligase CHFR antibody
    • EC 6.3.2. antibody
    • FLJ10796 antibody
    • FLJ33629 antibody
    • RING finger protein 196 antibody
    • RNF 116 antibody
    • RNF 196 antibody
    • RNF116 antibody
    • RNF196 antibody
    • Ubiquitin ligase protein antibody
    see all

Anti-CHFR antibody 画像

  • ab13773 (2.5 µg/ml) staining CHFR in Human Brain (Cerebellum) by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows stained Purkinje cells in Cerebellum.

Anti-CHFR antibody (ab13773) 使用論文

ab13773 has not yet been referenced specifically in any publications.

Product Wall

We will take back the goat antibody (ab13773) if you send it back at 4 deg C and cover the cost of the shipping plus a 20% re-stocking fee. A credit note for the original order minus the 20% re-stocking fee will be generated once the product is receive...

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The following protocol using ab13773 at 1 ug/ml and 0.5 ug/ml both gave a 80 kDa band in Human Brain lysates: - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Befo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"