製品の概要

  • 製品名
    Anti-CHES1 antibody [CHESC9H4]
    CHES1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CHESC9H4] to CHES1
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Recombinant fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant fragment

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a meium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    CHESC9H4
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

プロトコール

参考文献

This product has been referenced in:
  • Sun J  et al. The transcription factor FOXN3 inhibits cell proliferation by downregulating E2F5 expression in hepatocellular carcinoma cells. Oncotarget 7:43534-43545 (2016). WB . Read more (PubMed: 27259277) »
  • Huot G  et al. CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and protein biosynthesis. Mol Biol Cell 25:554-65 (2014). Read more (PubMed: 24403608) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 (Human liver carcinoma cell line))
Loading amount
40 µg
Specification
HepG2 (Human liver carcinoma cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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