製品の概要

  • 製品名Anti-Chemokine Receptor D6 antibody
    Chemokine Receptor D6 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Chemokine Receptor D6
  • 特異性Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • アプリケーション適用あり: Flow Cyt, ICC, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide:

    SSSIYDYDYLDDMTILVCRKDEVLSFGRC

    , corresponding to amino acids 18-45 of Mouse Chemokine Receptor D6

  • ポジティブ・コントロール
    • Mouse spleen cells or lung.
  • 特記事項


    Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. 1-3µl/106cells/50-100µl.

ICC 1/500.
IHC-P 1/250.
ELISA 1/100000.

ターゲット情報

  • 機能Receptor for C-C type chemokines including SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3.
  • 組織特異性Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C C chemokine receptor D6 antibody
    • C-C chemokine receptor D6 antibody
    • CC chemokine binding receptor JAB61 antibody
    • CC chemokine receptor D6 antibody
    • CCBP 2 antibody
    • CCBP2 antibody
    • CCBP2_HUMAN antibody
    • CCR 10 antibody
    • CCR 9 antibody
    • CCR10 antibody
    • CCR9 antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C) receptor 9 antibody
    • Chemokine binding protein 2 antibody
    • Chemokine binding protein D6 antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 9 antibody
    • Chemokine CC motif receptor 9 antibody
    • Chemokine CC receptor 9 antibody
    • Chemokine receptor 9 antibody
    • Chemokine receptor CCR 10 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR-10 antibody
    • Chemokine receptor CCR-9 antibody
    • Chemokine receptor CCR10 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine receptor D6 antibody
    • Chemokine-binding protein 2 antibody
    • Chemokine-binding protein D6 antibody
    • CMKBR 9 antibody
    • CMKBR9 antibody
    • D6 antibody
    • hD6 antibody
    • MGC126678 antibody
    • MGC138250 antibody
    see all

Anti-Chemokine Receptor D6 antibody 画像

  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse lung section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Chemokine Receptor D6 antibody (ab1656) 使用論文

This product has been referenced in:
  • Hajrasouliha AR  et al. Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection. Mol Vis 19:2517-25 (2013). Flow Cyt ; Mouse . Read more (PubMed: 24357920) »
  • Kin NW  et al. DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae. J Immunol 180:6663-74 (2008). WB, Flow Cyt ; Mouse . Read more (PubMed: 18453586) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Thank you for submitting an Abreview of ab1656. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species, we would like to follow up on this to see if we c...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 19 2012

Thank you for calling Abcam yesterday.
I have talked to the lab and I am sorry but we do not have an immunohistochemistry image showing the use of the blocking peptide with the antibody.
As we talked about yesterday as well, please find below...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy on lymphocytes
Username

Abcam user community

Verified customer

投稿 Dec 20 2011

Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from whole blood
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Formaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

投稿 Nov 08 2010

Thank you for your email and I'm sorry to hear that you are still experiencing difficulty with ab1656. You should definitely see positive staining with the spleen and lung tissue and so it seems as if the antibody is damaged in some way. I can have a r...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is rec...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is rec...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"