Mouse polyclonal to Chemerin
Full length Human Chemerin protein (NP_002880.1, 1 a.a. ~ 163 a.a).
Chemerin transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
Use at an assay dependent concentration.
Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.
Information by UniProt
RAR responsive protein TIG2 antibody
RAR-responsive protein TIG2 antibody
Western blot - Chemerin antibody (ab72965)
All lanes :
Anti-Chemerin antibody (ab72965) at 1/500 dilution
Lane 1 :
Chemerin transfected 293T cell lysate
Lane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size:
Observed band size:
15 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Chemerin antibody (ab72965)
ICC/IF image of ab72965 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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