Anti-CHD6 抗体 (ab114095)
Key features and details
- Rabbit polyclonal to CHD6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHD6 antibody
CHD6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CHD6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region between amino acids 2665- 2715 of Human CHD6 (NP_115597.3).
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ポジティブ・コントロール
- Whole cell lysate from Hela cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 305 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 305 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Probable transcription regulator. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 84181 Human
- SwissProt: Q8TD26 Human
- Unigene: 371979 Human
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別名
- ATP dependent helicase CHD6 antibody
- ATP-dependent helicase CHD6 antibody
- CHD-6 antibody
see all
画像
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All lanes : Anti-CHD6 antibody (ab114095) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 305 kDa
Exposure time: 30 seconds -
ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab114095 は 2 報の論文で使用されています。
- Zhang B et al. The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling. Cell Discov 8:130 (2022). PubMed: 36473865
- Xu G et al. Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5. Int J Mol Med 41:155-163 (2018). PubMed: 29115379