製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CHD4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CHD4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 218 kDa (predicted molecular weight: 218 kDa).

ターゲット情報

  • 機能
    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • 配列類似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

画像

  • Anti-CHD4 antibody (ab85620) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 218 kDa
    Observed band size : 218 kDa
    Additional bands at : 120 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

参考文献

ab85620 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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