This antibody gave a positive signal in HeLa (Human epithelial carcinoma cell line) nuclear lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Predicted band size: 218 kDa Observed band size: 218 kDa Additional bands at: 120 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Abcam recommends loading unheated lysates and using 20% Ethanol and 0.04% SDS in the transfer buffer to obtain a positive signal for this antibody. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above