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Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab70469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 218 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemical analysis of blastocysts from Wildtype and CHD4 -/- (Knockout) mice, labeling CHD4 with ab70469. Embryos were fixed in 2.5% paraformaldehyde, permeablised with 0.25% Triton X-100 and 3% polyvinlypyrrolidone in PBS, then blocked in 10% foetal calf serum and 0.1 Triton X-100 in PBS. Immunostaining with ab70469 (diluted 1/10,000) was performed overnight at 4°C. Staining with secondary antibody was for 1 hour at RT. DAPI used for nuclear staining (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"