Chromodomain helicase DNA binding protein 4 antibody
Chromodomain-helicase-DNA-binding protein 4 antibody
Mi 2 autoantigen 218 kDa protein antibody
Mi 2b antibody
Mi-2 autoantigen 218 kDa protein antibody
Mi2 beta antibody
Anti-CHD4 antibody [3F2/4] - ChIP Grade 画像
Western blot - Anti-CHD4 antibody [3F2/4] - ChIP Grade (ab70469)
All lanes : Anti-CHD4 antibody [3F2/4] - ChIP Grade (ab70469) at 5 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203) Lane 2 : Kidney (Mouse) Tissue Lysate Lane 3 : Human placenta tissue lysate - total protein (ab29745) Lane 4 : Human skin tissue lysate - total protein (ab30166) Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 218 kDa Additional bands at : 238 kDa. We are unsure as to the identity of these extra bands.
IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunoprecipitation - Anti-CHD4 antibody [3F2/4] - ChIP Grade (ab70469)This image is courtesy of an Abreview submitted by Dr Catia Andreassi
CHD4 immunoprecipitation from murine brain tissue lysate using ab70469. 5000 µg of cell lysate was incubated with primary antibody (undiluted) and matrix (protein G) and incubated for 14 hours.
An HRP-conjugated rabbit anti-CHD4 polyclonal (1/1000) was used to detect CHD4 immunoprecipitation.
O'Shaughnessy-Kirwan A et al. Constraint of gene expression by the chromatin remodelling protein CHD4 facilitates lineage specification. Development142:2586-97 (2015).
Read more (PubMed: 26116663) »
Singh AP & Archer TK Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation. Nucleic Acids Res42:2958-75 (2014).
Read more (PubMed: 24335282) »