The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
1/100 - 1/250.
Is unsuitable for IP.
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Chromodomain-helicase-DNA-binding protein 3 antibody
Mi 2 autoantigen 240 kDa protein antibody
Mi 2a antibody
Mi-2 autoantigen 240 kDa protein antibody
Mi2 ALPHA antibody
Prp9 1 antibody
Zinc finger helicase antibody
zinc-finger helicase (Snf2-like) antibody
Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
Predicted band size : 227 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: CHD3 knockout HAP1 cell lysate (20 µg) Lane 3: Raji cell lysate (20 µg) Lane 4: Y79 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa. ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - CHD3 antibody [EPNCIR110A] (ab109195)
All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution
Lane 1 : K562 cell lysate Lane 2 : HeLa cell lysate Lane 3 : C6 cell lysate Lane 4 : PC12 cell lysate