製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CHD3
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1950 and 2000 of Human CHD3 (NP_001005273.1).

  • ポジティブ・コントロール
    • Human Anaplastic Thyroid Carcinoma, Ewing Sarcoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma and Small Cell Lung Cancer tissue. Mouse Teratoma and Renal Cell Carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.

ターゲット情報

  • 機能
    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • 細胞内局在
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 226 antibody
    • 2600010P09Rik antibody
    • AF020312 antibody
    • ATP dependent helicase CHD3 antibody
    • ATP-dependent helicase Chd3 antibody
    • CHD-3 antibody
    • Chd3 antibody
    • CHD3_HUMAN antibody
    • Chd7 antibody
    • Chromodomain-helicase-DNA-binding protein 3 antibody
    • hZFH antibody
    • MGC40857 antibody
    • Mi 2 autoantigen 240 kDa protein antibody
    • Mi 2a antibody
    • Mi-2 autoantigen 240 kDa protein antibody
    • Mi2 ALPHA antibody
    • Mi2-alpha antibody
    • Prp7 antibody
    • Prp9 1 antibody
    • ZFH antibody
    • Zinc finger helicase antibody
    • zinc-finger helicase (Snf2-like) antibody
    see all

画像

  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry using DAB staining.
  • ab84528, at 1/250 dilution, staining CHD3 in formalin-fixed, paraffin-embedded mouse renal cell carcinoma by Immunohistochemistry using DAB staining.

プロトコール

参考文献

This product has been referenced in:
  • Yamashita D  et al. Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2a. J Biol Chem 291:11619-34 (2016). Read more (PubMed: 27068747) »
  • Klement K  et al. Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair. J Cell Biol 207:717-33 (2014). Read more (PubMed: 25533843) »

See all 2 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab84528.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録