製品の概要

  • 製品名Anti-CHD3 antibody
    CHD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CHD3
  • アプリケーション適用あり: IP, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1950-2000 of Human CHD3 (NP_001005273.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
    • 組織特異性Widely expressed.
    • 配列類似性Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 226 antibody
      • 2600010P09Rik antibody
      • AF020312 antibody
      • ATP dependent helicase CHD3 antibody
      • ATP-dependent helicase Chd3 antibody
      • CHD-3 antibody
      • Chd3 antibody
      • CHD3_HUMAN antibody
      • Chd7 antibody
      • Chromodomain-helicase-DNA-binding protein 3 antibody
      • hZFH antibody
      • MGC40857 antibody
      • Mi 2 autoantigen 240 kDa protein antibody
      • Mi 2a antibody
      • Mi-2 autoantigen 240 kDa protein antibody
      • Mi2 ALPHA antibody
      • Mi2-alpha antibody
      • Prp7 antibody
      • Prp9 1 antibody
      • ZFH antibody
      • Zinc finger helicase antibody
      • zinc-finger helicase (Snf2-like) antibody
      see all

    Anti-CHD3 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ewing sarcoma (left) and mouse renal cell carcinoma (right) tissues labelling CHD3 with ab114100 at 1/1000 (1µg/ml). Detection: DAB.
    • Rabbit anti-CHD3 antibody, at 1 µg/ml, staining CHD3 in HeLa whole cell lysate immunoprecipitated using ab114100 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-CHD3 antibody (ab114100) 使用論文

    ab114100 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"