ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin.
Localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis
Colonic and hepatic tumor over expressed protein antibody
Colonic and hepatic tumor overexpressed protein antibody
Cytoskeleton associated protein 5 antibody
Western blot - Anti-ch TOG antibody (ab86073)
All lanes : Anti-ch TOG antibody (ab86073) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 218 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-ch TOG antibody (ab86073)
Immunoprecipitation/ Western Blot of ab86073. Lane 1: ab86073 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86073 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.