製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CGRP
  • 特異性
    Antibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
  • アプリケーション
    適用あり: IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
    適用なし: IHC-R
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat CGRP (C terminal).
    Sequence:

    VKDNFVPTNVGSEAF

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent dilution. PubMed: 19805319
ELISA 1/500 - 1/2500.
IHC-P 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
  • 追加情報
    Is unsuitable for IHC-R.
  • ターゲット情報

    • 関連性
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • 細胞内局在
      Endoplasmic reticulum and Secreted
    • 参照データベース
    • 別名
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Anti-CGRP antibody 画像

    • IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.

      See Abreview

    • ab36001 staining mouse colon tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C.  The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.

      See Abreview

    プロトコール

    Anti-CGRP antibody (ab36001) 使用論文

    This product has been referenced in:
    • Deiteren A  et al. P2X3 receptors mediate visceral hypersensitivity during acute chemically-induced colitis and in the post-inflammatory phase via different mechanisms of sensitization. PLoS One 10:e0123810 (2015). IHC-FoFr ; Rat . Read more (PubMed: 25885345) »
    • Mathews MA  et al. Efferent Vestibular Neurons Show Homogenous Discharge Output But Heterogeneous Synaptic Input Profile In Vitro. PLoS One 10:e0139548 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26422206) »

    See all 20 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Dorsal Root Ganglia)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dorsal Root Ganglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Jean-Marc Guedon

    Verified customer

    投稿 Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Feb 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Sep 06 2012

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, spinal cord, muscle w/nerve)
    Specification
    brain, spinal cord, muscle w/nerve
    Fixative
    formalin, 10% neutral buffered
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6, microwave 2x, 5 min on high
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 18 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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