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This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
Our Abpromise guarantee covers the use of ab81887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24430801
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ab81887 staining rat DRG sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde, permeabilised in 0.3% PBS-Tween and blocked for non-specific protein-protein interactions with 10% donkey's serum for 1h at 24°C. Staining with ab81887 at a 1/1000 dilution in PBS-Triton (0.3%) with 10% donkey's serum and 0.02% azide was performed for 24h at 4°C. A donkey anti-mouse Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.
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