製品の概要

  • 製品名Anti-CG18321 antibody
  • 製品の詳細
    Mouse polyclonal to CG18321
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 57-156 of Fruit fly (Drosophila melanogaster) CG18321. Target sequence used to make the antibody: AETVASTTNS DTHRTRNNRK PNKPQEHSQQ VGAHKSGSRK LVVAENGGAL SSQLPQPNHK QGHKNTDKQQ RGGGSKQQRG HSQHHGGNRK GPKAATPENG.

  • 特記事項

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 30 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性CG18321 (miple2) is a member of the MK/PTN family of proteins. Drosophila Miple1 and miple2 are secreted proteins which are expressed in spatially restricted, nonoverlapping patterns during embryogenesis. CG18321 is strongly expressed in the developing midgut endoderm. The exact molecular functions and biological processes in which it is involved are not known. Mutations have been isolated which are viable and fertile.
  • 参照データベース
    • 別名
      • Miple2 antibody
      • RE13914p antibody

    Anti-CG18321 antibody 画像

    • All lanes : Anti-CG18321 antibody (ab52682) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (tagged antigen fusion protein).

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

      Predicted band size : 30 kDa

    Anti-CG18321 antibody (ab52682) 使用論文

    ab52682 has not yet been referenced specifically in any publications.

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