Anti-Ceruloplasmin 抗体 (ab110449)


  • 製品名Anti-Ceruloplasmin antibody
    Ceruloplasmin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ceruloplasmin
  • アプリケーション適用あり: IHC-FoFr, WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide within Human Ceruloplasmin aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab111803)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human plasma total protein lysate as well as Human liver tissue lysate.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab110449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 122 kDa).
ICC/IF Use at an assay dependent concentration.


  • 機能Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 配列類似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

Anti-Ceruloplasmin antibody 画像

  • ab110449 staining ceruloplasmin in astroglial cells from rat by ICC/IF (Immunocytochemistry/immunofluorescence).  Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1% PBS and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody for 4 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Donkey polyclonal was used as the secondary antibody.


  • Adult rat was perfused with 4% PFA in 0.1M PBS. Fluorescent staining was performed in duodenum sections with anti-Ceruloplasmin (ab110449) primary and Alexa 488 conjugated secondary antibody. This antibody stained gut enterocytes. Nucleus stained with Hoechst at high power magnification.

    See Abreview

  • All lanes : Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 148 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,75 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Natural Human Ceruloplasmin protein (ab77930) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa

    Exposure time : 30 seconds

Anti-Ceruloplasmin antibody (ab110449) 使用論文

ab110449 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your comment.

Before offering a testing discount for new species, our Scientific Team need to check the sequence homology to see if the cross-reactivity is expected or not.

If the homology is higher than 95%, then ther...

Read More
Application Western blot
Sample Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions Reduced Denaturing (8)
Loading amount 30 µg
Specification endometrial tissue lysate
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jun 26 2015

Thank you for the reply.

I have checked the sequence homology between human and pig (Sus scrofa) and it is only 87%. This means that the probability of this antibody to recognize pig (porcine) Ceruloplasmin is low. Please advise your customer...

Read More

Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Duodenum)
Specification Duodenum

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astro-glia)
Specification Astro-glia
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 02 2011