製品の概要

  • 製品名
    Anti-Ceruloplasmin antibody [3B11]
    Ceruloplasmin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3B11] to Ceruloplasmin
  • アプリケーション
    適用あり: IHC-P, ChIP, WB, IP, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein purified from Human plasma

  • ポジティブ・コントロール
    • Human plasma
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR162822 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, PBS
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    3B11
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 122 kDa.
IP Use at an assay dependent concentration.

Use 2μg

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 組織特異性
    Expressed by the liver and secreted in plasma.
  • 関連疾患
    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 配列類似性
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

画像

  • All lanes : Anti-Ceruloplasmin antibody [3B11] (ab51083) at 1/1000 dilution

    Lane 1 : Ceruloplasmin isolated from human plasma
    Lane 2 : Human plasma


    Predicted band size : 122 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Guo Z  et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . Read more (PubMed: 26608305) »
  • Liu X  et al. An individual urinary proteome analysis in normal human beings to define the minimal sample number to represent the normal urinary proteome. Proteome Sci 10:70 (2012). WB . Read more (PubMed: 23170922) »

See all 3 Publications for this product

レビューと Q&A


The lab let me know that the immunogen of ab51083 is a protein purified from Human plasma.
And thus, the protein sequence is full and has not been mapped. This would apply also for the epitope which has not been mapped either.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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