製品の概要

  • 製品名Anti-CERKL antibody
    CERKL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CERKL
  • 特異性This antibody detects endogenous levels of total CERKL protein.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthesized peptide derived from the internal region of human CERKL.

  • ポジティブ・コントロール
    • Extracts from A549 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 63, 40 and 48 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
    • 組織特異性Isoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver.
    • 関連疾患Defects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive.
    • 配列類似性Contains 1 DAGKc domain.
    • 発生段階Expressed in fetal lung, kidney and brain.
    • 翻訳後修飾Phosphorylated on serine residues.
    • 細胞内局在Cytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ceramide kinase like protein antibody
      • Ceramide kinase-like protein antibody
      • CERKL antibody
      • CERKL_HUMAN antibody
      • Retinitis pigmentosa 26 (autosomal recessive) antibody
      • RP26 antibody
      see all

    Anti-CERKL antibody 画像

    • All lanes : Anti-CERKL antibody (ab66007) at 1/500 dilution

      Lane 1 : Extracts from A549 cells
      Lane 2 : Extracts from A549 cells, plus 5µg immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 63 kDa
      Additional bands at : 40 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Anti-CERKL antibody (ab66007) 使用論文

    This product has been referenced in:
    • Jelcick AS  et al. Genetic variations strongly influence phenotypic outcome in the mouse retina. PLoS One 6:e21858 (2011). WB ; Mouse . Read more (PubMed: 21779340) »

    See 1 Publication for this product

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