製品の概要

  • 製品名
    Anti-Ceramide glucosyltransferase antibody
    Ceramide glucosyltransferase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ceramide glucosyltransferase
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Ceramide glucosyltransferase.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能
    Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
  • 組織特異性
    Found in all tissues examined.
  • パスウェイ
    Lipid metabolism; sphingolipid metabolism.
  • 配列類似性
    Belongs to the glycosyltransferase 2 family.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CEGT_HUMAN antibody
    • Ceramide glucosyltransferase antibody
    • GCS antibody
    • GLCT 1 antibody
    • GLCT-1 antibody
    • GLCT1 antibody
    • Glucosylceramide synthase antibody
    • UDP glucose ceramide glucosyltransferase antibody
    • UDP glucose N acylsphingosine D glucosyltransferase antibody
    • UDP-glucose ceramide glucosyltransferase antibody
    • UDP-glucose:N-acylsphingosine D-glucosyltransferase antibody
    • Ugcg antibody
    see all

画像

  • All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Spleen (Mouse) Tissue Lysate
    Lane 5 : Spleen (Rat) Tissue Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,36 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

参考文献

This product has been referenced in:
  • Sun R  et al. Lowered humidity produces human epidermal equivalents with enhanced barrier properties. Tissue Eng Part C Methods 21:15-22 (2015). Read more (PubMed: 24803151) »

See 1 Publication for this product

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