製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CEP78
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 639 and 689 of human CEP78 (CAI40237.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats.
    • 細胞内局在
      Cytoplasmic; centrosome
    • 参照データベース
    • 別名
      • 5730599I05Rik antibody
      • C9orf81 antibody
      • Centrosomal protein 78 antibody
      • Centrosomal protein 78kDa antibody
      • Chromosome 9 open reading frame 81 antibody
      • D030027P05 antibody
      • FLJ12643 antibody
      • FLJ52093 antibody
      • IP63 antibody
      • MGC135040 antibody
      • RP11-336N8.5 antibody
      see all

    画像

    • All lanes : Anti-CEP78 antibody (ab86188) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 76 kDa
      Observed band size : 76 kDa


      Exposure time : 3 minutes
    • Detection of human CEP78 in Immunoprecipitates of HeLa whole cell lysate (1 mg klysate for IP, 20% of IP loaded) using ab86188 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    参考文献

    ab86188 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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