Rabbit polyclonal to CEP76
Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Use at 10 µg/mg of lysate.
Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
Belongs to the CEP76 family.
Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
Information by UniProt
Centrosomal protein 76kDa antibody
Centrosomal protein of 76 kDa antibody
Western blot - Anti-CEP76 antibody (ab86613)
All lanes :
Anti-CEP76 antibody (ab86613) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size:
Observed band size:
69 kDa (
why is the actual band size different from the predicted?
Additional bands at:
115 kDa, 305 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 3 minutes
Immunoprecipitation - Anti-CEP76 antibody (ab86613)
Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds All lanes : Anti-CEP76 antibody (ab86613) at 1 µg/ml Lane 1 : immunoprecipitated CEP76 Lane 2 : control IgG
has not yet been referenced specifically in any publications.
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