製品の概要

  • 製品名
    Anti-CEP76 antibody
  • 製品の詳細
    Rabbit polyclonal to CEP76
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP Use at 10 µg/mg of lysate.

ターゲット情報

  • 機能
    Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
  • 配列類似性
    Belongs to the CEP76 family.
  • 発生段階
    Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C18orf9 antibody
    • Centrosomal protein 76kDa antibody
    • Centrosomal protein of 76 kDa antibody
    • CEP 76 antibody
    • Cep76 antibody
    • CEP76_HUMAN antibody
    • Chromosome 18 open reading frame 9 antibody
    • FLJ12542 antibody
    • HsT1705 antibody
    see all

画像

  • All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 76 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa,305 kDa. We are unsure as to the identity of these extra bands.
  • Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 30 seconds

参考文献

ab86613 has not yet been referenced specifically in any publications.

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