製品の概要

  • 製品名Anti-CEP68 antibody
    CEP68 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CEP68
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 350-400 (PTNVSPNCPP AEATALPFSG PREPSLKQWP SRVPQKQGGM GLASWSQLAS T) of Human CEP68 (NP_055962.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was affinity purified using an epitope specific to CEP68 immobilized on a solid support
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 5-15 µg/mg of lysate.

ターゲット情報

Anti-CEP68 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling CEP68 with ab91460 at 1/1000 (1µg/ml). Detection: DAB.
  • ab91460 at 1 µg/ml detecting CEP68 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91460 at 10 µg/mg of lysate (lane 2), CEP68 was also immunoprecipitated by an antibody to an upstream epitope of CEP68 (lane 1) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
  • All lanes : Anti-CEP68 antibody (ab91460) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 81 kDa


    Exposure time : 3 minutes

Anti-CEP68 antibody (ab91460) 使用論文

ab91460 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO)
Specification CHO
Permeabilization No
Fixative Methanol
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投稿 Dec 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"