Anti-Centrin 2 with anti-GAPDH internal loading control 抗体 (ab80863)


  • 製品名Anti-Centrin 2 with anti-GAPDH internal loading control antibody
  • 製品の詳細
    Rabbit polyclonal to Centrin 2 with anti-GAPDH internal loading control
  • アプリケーション適用あり: WBmore details
    適用なし: ChIP,Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Centrin 2 with anti-GAPDH internal loading control.

  • ポジティブ・コントロール
    • This cocktail gave a positive signal in Jurkat whole cell lysate.



Our Abpromise guarantee covers the use of ab80863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19, 37 kDa (predicted molecular weight: 19, 37 kDa). ab80863 contains 2 antibodies, generated by raising against a Centrin 2 immunogen and apiking with anti-GAPDH loading control.
  • 追加情報Is unsuitable for ChIP,Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Plays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110.
      Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
      The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
    • 配列類似性Belongs to the centrin family.
      Contains 4 EF-hand domains.
    • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus. Centrosome of S-phase, interphase and mitotic cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CALT antibody
      • Caltractin (20kD calcium-binding protein) antibody
      • Caltractin isoform 1 antibody
      • CEN2 antibody
      • Centrin EF hand protein 2 antibody
      • Centrin-2 antibody
      • CETN2 antibody
      • CETN2_HUMAN antibody
      see all

    Anti-Centrin 2 with anti-GAPDH internal loading control antibody 画像

    • Anti-Centrin 2 with anti-GAPDH internal loading control antibody (ab80863) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 19, 37 kDa
      Observed band size : 19,37 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    Anti-Centrin 2 with anti-GAPDH internal loading control antibody (ab80863) 使用論文

    ab80863 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab80863.
    Please use the links above to contact us or submit feedback about this product.