Anti-Centrin 2 with anti-GAPDH internal loading control 抗体 (ab80863)
Key features and details
- Rabbit polyclonal to Centrin 2 with anti-GAPDH internal loading control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Centrin 2 with anti-GAPDH internal loading control antibody -
製品の詳細
Rabbit polyclonal to Centrin 2 with anti-GAPDH internal loading control -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ChIP,Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Centrin 2 with anti-GAPDH internal loading control aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab138815) -
ポジティブ・コントロール
- This cocktail gave a positive signal in Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80863の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 19, 37 kDa (predicted molecular weight: 19, 37 kDa). ab80863 contains 2 antibodies, generated by raising against a Centrin 2 immunogen and apiking with anti-GAPDH loading control.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19, 37 kDa (predicted molecular weight: 19, 37 kDa). ab80863 contains 2 antibodies, generated by raising against a Centrin 2 immunogen and apiking with anti-GAPDH loading control. |
ターゲット情報
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機能
Plays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. -
配列類似性
Belongs to the centrin family.
Contains 4 EF-hand domains. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus. Centrosome of S-phase, interphase and mitotic cells. - Information by UniProt
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参照データベース
- Entrez Gene: 1069 Human
- Entrez Gene: 26370 Mouse
- Entrez Gene: 84593 Rat
- Omim: 300006 Human
- SwissProt: Q2TBN3 Cow
- SwissProt: P41208 Human
- SwissProt: Q9R1K9 Mouse
- Unigene: 82794 Human
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別名
- CALT antibody
- Caltractin (20kD calcium-binding protein) antibody
- Caltractin isoform 1 antibody
see all
画像
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Anti-Centrin 2 with anti-GAPDH internal loading control antibody (ab80863) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19, 37 kDa
Observed band size: 19,37 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The 19 and 37 kDa bands correlate to Centrin 2 and GAPDH, respectively.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab80863 は 1 報の論文で使用されています。
- Ramenzoni LL et al. Periodontal bacterial supernatants modify differentiation, migration and inflammatory cytokine expression in human periodontal ligament stem cells. PLoS One 14:e0219181 (2019). PubMed: 31269072