Centrin 2 overexpression 293T lysate (whole cell) (ab94332)

製品の概要

  • 製品名Centrin 2 overexpression 293T lysate (whole cell)
  • 製品の詳細
    Centrin 2 overexpression lysate
  • 特記事項ab94332 is a 293T cell transfected lysate in which Human Centrin 2 has been transiently over-expressed using a pCMV-Centrin 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Plays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. Similarity: Belongs to the centrin family. Contains 4 EF-hand domains.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Centrin 2 overexpression 293T lysate (whole cell) 画像

  • ab94332 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Centrin 2 antibody (ab54525) at 1/500 dilution

    Lane 1 : Centrin 2 overexpression 293T lysate (whole cell) (ab94332)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Centrin 2 overexpression 293T lysate (whole cell) (ab94332) 使用論文

ab94332 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"