製品の概要

  • 製品名Anti-Centrin 1 antibody
    Centrin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Centrin 1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Dog, Human
    交差が予測される動物種: Xenopus laevis, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 157-172 of Human Centrin 1, with N-terminally added lysine. This sequence is highly conserved in centrin 1 of many species, e.g. mouse, drosophila, and Xenopus. It is also found in human and mouse centrin 2, but is not found in centrin 3.

  • ポジティブ・コントロール
    • HeLa cell line.
  • 特記事項Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Anti-centrin is affinity purified using the immunizing peptide immobilized on agarose.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 21 kDa. 1/2000, this concentration is recommended using a nuclear extract of the human epitheloid carcinoma HeLa cell line. Predicted molecular weight: 21 kDa.
ICC/IF 1/200. PubMed: 17299043

Suggested working dilution of 1/200 (see PMID: 17299043).

Methanol fixation may be preferable for this antibody based on publications and customer feedback.

ターゲット情報

  • 機能Plays a fundamental role in microtubule-organizing center structure and function.
  • 配列類似性Belongs to the centrin family.
    Contains 4 EF-hand domains.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Centrosome of interphase and mitotic cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Caltractin antibody
    • Caltractin isoform 2 antibody
    • CEN 1 antibody
    • CEN1 antibody
    • Centrin EF hand protein 1 antibody
    • Centrin-1 antibody
    • Centrin1 antibody
    • CETN 1 antibody
    • CETN antibody
    • CETN1 antibody
    • CETN1_HUMAN antibody
    • EF hand protein 1 antibody
    see all

Anti-Centrin 1 antibody 画像

  • ab11257 staining Centrin 1 in mouse embryonic fibroblast cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with Ethanol (-200C) and blocking with 3% BSA/PBS  was done for 30 minutes at 220C. Samples were incubated with primary antibody (1/500) in in 3% BSA /0.1 % saponin/PBS for 1 hour at RT. An undiluted Alexa Fluor®546-conjugated donkey polyclonal to goat IgG (H&L) was used at dilution at 1/500 as secondary antibody. In the image, Green staining represents gamma tubulin, red - centrin.

    See Abreview

Anti-Centrin 1 antibody (ab11257) 使用論文

This product has been referenced in:
  • Mukhopadhyay A  et al. 14-3-3? Prevents Centrosome Amplification and Neoplastic Progression. Sci Rep 6:26580 (2016). ICC/IF . Read more (PubMed: 27253419) »
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »

See all 13 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (gastrocoel roof plate)
Permeabilization Yes - 0.1% Triton X-100
Specification gastrocoel roof plate
Blocking step 3% BSA + 3% serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Sep 29 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Sample Mouse Cell (Liver)
Specification Liver
Permeabilization Yes - 0.5% Triton in PBS
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 13 2014

Thank you for your response.

I am not sure what IB represents but assume the customer would mean either IP (Immunoprecipitation) or WB (Western blot) application? Is that correct?

I regret to inform you that I am not convinced that ...

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Thank you for your response.

Unfortunately, the two image files can't be opened so I am unable to take a look at the signal.

Tested and Guaranted Applications:

ab11257 - WB, ICC/IF

ab99337 - WB and IP - this has ...

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Thank you for your enquiry regarding our two products. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on th...

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Thank you very much for your recent review of ab11257. Your review is now published on our website.
Since the results were below average, I'm contacting you to see if we can help resolve this problem. I would recommend reducing the amount of prote...

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 50 µg
Specification Hela
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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投稿 Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic stem)
Specification Embryonic stem
Fixative Methanol
Permeabilization Yes - 0.1% triton-X-100
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
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Prof. Reiter Lab

Verified customer

投稿 Apr 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse embryonic fibroblast (MEF))
Specification mouse embryonic fibroblast (MEF)
Fixative EtOH (-20C)
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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投稿 Mar 22 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"