製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CENPT
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 520-561 of Human CENPT (NP_079358.3 ).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 10 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
    • 配列類似性
      Belongs to the CENPT family.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C16orf56 antibody
      • CENP T antibody
      • CENP-T antibody
      • Cenpt antibody
      • CENPT_HUMAN antibody
      • Centromere protein T antibody
      • Chromosome 16 open reading frame 56 antibody
      • ICEN22 antibody
      • Interphase centromere complex protein 22 antibody
      see all

    画像

    • Detection of CENPT by Western Blot of Immunprecipitate.
      Lane 1: An antibody to CENPT at 1µg/ml staining CENPT in HeLa whole cell lysate immunoprecipitated using ab114120 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 3 seconds.

    プロトコール

    参考文献

    ab114120 has not yet been referenced specifically in any publications.

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