製品の概要

  • 製品名
    Anti-CENPN antibody
  • 製品の詳細
    Rabbit polyclonal to CENPN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 309-339 of Human BM039.

  • ポジティブ・コントロール
    • MCF-7 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 40 kDa.

ターゲット情報

  • 機能
    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BM 039 antibody
    • BM 309 antibody
    • BM-309 antibody
    • BM039 antibody
    • C16orf60 antibody
    • CENP N antibody
    • CENP-N antibody
    • Cenpn antibody
    • CENPN_HUMAN antibody
    • Centromere protein N antibody
    • FLJ13607 antibody
    • FLJ22660 antibody
    • ICEN 32 antibody
    • ICEN32 antibody
    • Interphase centromere complex protein 32 antibody
    see all

画像

  • Anti-CENPN antibody (ab104296) at 1/50 dilution + MCF7 cell lysate at 35 µg

    Predicted band size : 40 kDa

プロトコール

参考文献

ab104296 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Dec 08 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録