製品の概要

  • 製品名Anti-CENPH antibody
    CENPH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CENPH
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human CENPH protein (AAH12024) with a 26 kDa tag.

  • ポジティブ・コントロール
    • CENPH transfected 293T cell lysate; immunogen

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • 配列類似性Belongs to the centromere protein H family.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP H antibody
    • CENP-H antibody
    • CENPH antibody
    • CENPH_HUMAN antibody
    • Centromere protein H antibody
    • ICEN35 antibody
    • Interphase centromere complex protein 35 antibody
    • Kinetochore protein CENP H antibody
    • NNF1 antibody
    • NNF1, MIND kinetochore complex component, homolog antibody
    • PMF1 antibody
    see all

Anti-CENPH antibody 画像

  • Anti-CENPH antibody (ab77207) at 1/500 dilution + recombinant tagged human CENPH protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 28 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CENPH antibody (ab77207) at 1/500 dilution

    Lane 1 : CENPH transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-CENPH antibody (ab77207) 使用論文

ab77207 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77207.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"