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RabMAb

Anti-CENPE 抗体 [EPR4543(2)] (ab124733)

製品の概要

  • 製品名
    Anti-CENPE antibody [EPR4543(2)]
    CENPE 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4543(2)] to CENPE
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human CENPE.

  • ポジティブ・コントロール
    • HepG2, HeLa, and Jurkat cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 320 kDa (predicted molecular weight: 316 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 関連性
      CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation.
    • 細胞内局在
      Associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
    • 参照データベース
    • 別名
      • CENP E antibody
      • Centromere associated protein E antibody
      • Centromere autoantigen E (312kD) antibody
      • Centromere autoantigen E antibody
      • Centromere protein E 312kDa antibody
      • Centromere protein E antibody
      • KIF10 antibody
      • Kinesin family member 10 antibody
      • Kinesin related protein antibody
      • Kinesin related protein CENPE antibody
      • PPP1R61 antibody
      • Protein phosphatase 1, regulatory subunit 61 antibody
      see all

    画像



    • Predicted band size : 316 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124733 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124733 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab124733 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CENPE antibody [EPR4543(2)] (ab124733) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 316 kDa

    プロトコール

    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Aug 25 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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