Anti-CENPC 抗体 [2159C5a] (ab50974)
Key features and details
- Mouse monoclonal [2159C5a] to CENPC
- Suitable for: WB, Dot blot, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-CENPC antibody [2159C5a]
CENPC 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2159C5a] to CENPC -
由来種
Mouse -
アプリケーション
適用あり: WB, Dot blot, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CENPC aa 655-768.
Sequence:KPQTSGYTCNIPTESNLDSGEHKTSVLEESGPSRLNNNYLMSGKNDVDDE EVHGSSDDSKQSKVIPKNRIHHKLVLPSNTPNVRRTKRTRLKPLEYWRGE RIDYQGRPSGGFVI
Database link: Q03188 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
2159C5a -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration.
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|
Dot blot |
Use at an assay dependent concentration.
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|
ICC/IF | (3) |
Use at an assay dependent concentration.
Use at a dilution of 1/250 (for overnight incubation). |
特記事項 |
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WB
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. Use at a dilution of 1/250 (for overnight incubation). |
ターゲット情報
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機能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions. -
配列類似性
Belongs to the CENPC family. -
ドメイン
The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. - Information by UniProt
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参照データベース
- Entrez Gene: 1060 Human
- Omim: 117141 Human
- SwissProt: Q03188 Human
- Unigene: 479867 Human
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別名
- CENP-C 1 antibody
- CENP-C antibody
- CENPC_HUMAN antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (16)
ab50974 は 16 報の論文で使用されています。
- Chou HC et al. The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure. Elife 10:N/A (2021). PubMed: 33522487
- Chan DY et al. Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons. Exp Cell Res 388:111840 (2020). PubMed: 31930965
- Zielinska AP et al. Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs. Curr Biol 29:3749-3765.e7 (2019). PubMed: 31679939
- Nomani A et al. Evaluation of genotoxicity and mutagenic effects of vector/DNA nanocomplexes in transfected mesenchymal stem cells by flow cytometry. Acta Biomater 74:236-246 (2018). PubMed: 29783088
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633