Anti-CENPA 抗体 (ab65678)

製品の概要

• 製品名
  Anti-CENPA antibody
  CENPA 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to CENPA
• アプリケーション
  適用あり: IHC-P, WBmore details
• 種交差性
  交差種: Human
  交差が予測される動物種: Mouse, Rat
• 免疫原

  Synthetic peptide: MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE, corresponding to a region within N terminal amino acids 1-50 of Human CENPA

  Run BLAST with Run BLAST with
• ポジティブ･コントロール
  • Jurkat cell lysate. Human skin.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• バッファー
  Preservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• 精製度
  Protein A purified
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Epigenetics and Nuclear Signaling
  • Histones
  • Variants

  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Centromeres

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Centromere

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • Jurkat nuclear extract lysate (ab14844)

アプリケーション

ターゲット情報

• 機能
  Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
• 配列類似性
  Belongs to the histone H3 family.
• ドメイン
  The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
• 翻訳後修飾
  Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
  Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
• 細胞内局在
  Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.

• Target information above from: UniProt accession P49450 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 1058 Human
  • Entrez Gene: 12615 Mouse
  • Entrez Gene: 298850 Rat
  • Omim: 117139 Human
  • SwissProt: P49450 Human
  • SwissProt: O35216 Mouse
  • Unigene: 1594 Human
  • Unigene: 290563 Mouse

  • Unigene: 16165 Rat

  see all
• 別名
  • CENP A antibody
  • CENP-A antibody
  • cenpa antibody

  • CENPA_HUMAN antibody
  • Centromere autoantigen A antibody
  • Centromere protein A 17kDa antibody
  • Centromere protein A antibody
  • Histone H3 like centromeric protein A antibody
  • Histone H3-like centromeric protein A antibody

  see all

プロトコール

• Western blot protocols
• Immunohistochemistry protocols