製品の概要
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製品名Anti-CENPA antibody
CENPA 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CENPA
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide: MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE, corresponding to a region within N terminal amino acids 1-50 of Human CENPA
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ポジティブ･コントロール
- Jurkat cell lysate. Human skin.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab65678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 4 - 8 µg/ml. | |
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
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配列類似性Belongs to the histone H3 family.
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ドメインThe CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
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翻訳後修飾Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12. -
細胞内局在Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
- Information by UniProt
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参照データベース
- Entrez Gene: 1058 Human
- Entrez Gene: 12615 Mouse
- Entrez Gene: 298850 Rat
- Omim: 117139 Human
- SwissProt: P49450 Human
- SwissProt: O35216 Mouse
- Unigene: 1594 Human
- Unigene: 290563 Mouse
see all -
別名
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
画像
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Western blot - Anti-CENPA antibody (ab65678)Anti-CENPA antibody (ab65678) at 1.25 µg/ml + Jurkat cell lysate
Predicted band size: 16 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody (ab65678)Immunohistochemical analysis of paraffin-embedded human skin tissue stained with ab65678 at 4µg/ml. Positively stained squamous epithelial cells are indicated with arrows.
参考文献
ab65678 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"