製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CENPA
  • 特異性
    Detects endogenous levels of total CENPA protein.
  • アプリケーション
    適用あり: ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human CENPA around the phosphorylation site of serine 7 (R-R-SP-R-K).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • 配列類似性
    Belongs to the histone H3 family.
  • ドメイン
    The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
  • 翻訳後修飾
    Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
    Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP A antibody
    • CENP-A antibody
    • cenpa antibody
    • CENPA_HUMAN antibody
    • Centromere autoantigen A antibody
    • Centromere protein A 17kDa antibody
    • Centromere protein A antibody
    • Histone H3 like centromeric protein A antibody
    • Histone H3-like centromeric protein A antibody
    see all

画像

  • ab62688 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells (treated with Forskolin (40nM, 30mins) or untreated) by Immunofluorescence,

参考文献

ab62688 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab62688.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録