Anti-CEND1 抗体 [EPR3739] (ab113076)

製品の概要

  • 製品名Anti-CEND1 antibody [EPR3739]
    CEND1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3739] to CEND1
  • アプリケーション適用あり: WB, IHC-P, ICC, IP, IHC-Frmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CEND1.

  • ポジティブ・コントロール
    • Human fetal brain lysate, U-87 MG and Neuro 2a cell lysates; Human brain glioma tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
ICC 1/250 - 1/500.
IP 1/50.
IHC-Fr Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    Anti-CEND1 antibody [EPR3739] 画像

    • All lanes : Anti-CEND1 antibody [EPR3739] (ab113076) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG cell lysate
      Lane 3 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human brain tissue by Immunohistochemistry.
    • ab113076 at 1/500 dilution, staining CEND1 in Praffin-embedded Human glioma tissue by Immunohistochemistry.
    • ab113076 staining CEND1 in Rat retina (at birth) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 24 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    Anti-CEND1 antibody [EPR3739] (ab113076) 使用論文

    ab113076 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Rat Tissue sections (retina of rat at birth)
    Specification retina of rat at birth
    Permeabilization Yes - 0.1% Triton
    Fixative Formaldehyde
    Username

    Mrs. Claudine Botteri

    Verified customer

    投稿 Feb 25 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"