Anti-Cellular Apoptosis Susceptibility 抗体 (ab70547)

製品の概要

  • 製品名
    Anti-Cellular Apoptosis Susceptibility antibody
    Cellular Apoptosis Susceptibility 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cellular Apoptosis Susceptibility
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide mapping to a region between residues 1 and 50 of human Cellular Apoptosis Susceptibility using the numbering given in entry NP_001307.2

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and Ramos cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 組織特異性
    Highly expressed in proliferating cells.
  • 配列類似性
    Belongs to the XPO2/CSE1 family.
    Contains 1 importin N-terminal domain.
  • 細胞内局在
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAS antibody
    • Cellular apoptosis susceptibility protein antibody
    • Chromosome segregation 1 (yeast homolog) like antibody
    • Chromosome segregation 1 Like antibody
    • Chromosome segregation 1 like protein antibody
    • Chromosome segregation 1-like protein antibody
    • Chromosome segregation gene CSE1 antibody
    • CSE 1 antibody
    • CSE 1 chromosome segregation 1 like antibody
    • CSE 1 chromosome segregation 1 like protein antibody
    • CSE 1L antibody
    • CSE1 antibody
    • CSE1 chromosome segregation 1 like (yeast) antibody
    • CSE1 chromosome segregation 1 like antibody
    • CSE1 chromosome segregation 1 like protein antibody
    • CSE1L antibody
    • Exp 2 antibody
    • Exp2 antibody
    • Exportin 2 antibody
    • Exportin-2 antibody
    • Exportin2 antibody
    • Importin alpha re exporter antibody
    • Importin-alpha re-exporter antibody
    • MGC117283 antibody
    • MGC130036 antibody
    • MGC130037 antibody
    • XPO 2 antibody
    • XPO2 antibody
    • XPO2_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) labelling Cellular Apoptosis Susceptibility with ab70547 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab70547) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from Ramos cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 3 minutes
  • 1mg whole cell lysate was immunoprecipitated using ab70547 at 3ug/ml (lane 1) or a control IgG (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane and probed with ab70547 at 1ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.

参考文献

ab70547 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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