Anti-Cellular Apoptosis Susceptibility 抗体 (ab54674)


  • 製品名Anti-Cellular Apoptosis Susceptibility antibody
    Cellular Apoptosis Susceptibility 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Cellular Apoptosis Susceptibility
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: LIGLFELPED DTIPDEEHFI DIEDTPGYQT AFSQLAFAGK KEHDPVGQMV NNPKIHLAQS LHKLSTACPG RVPSMVSTSL NAEALQYLQG YLQAASVTLL , corresponding to amino acids 872-972 of Human Cellular Apoptosis Susceptibility



Our Abpromise guarantee covers the use of ab54674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 機能Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 組織特異性Highly expressed in proliferating cells.
  • 配列類似性Belongs to the XPO2/CSE1 family.
    Contains 1 importin N-terminal domain.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAS antibody
    • Cellular apoptosis susceptibility protein antibody
    • Chromosome segregation 1 (yeast homolog) like antibody
    • Chromosome segregation 1 Like antibody
    • Chromosome segregation 1 like protein antibody
    • Chromosome segregation 1-like protein antibody
    • Chromosome segregation gene CSE1 antibody
    • CSE 1 antibody
    • CSE 1 chromosome segregation 1 like antibody
    • CSE 1 chromosome segregation 1 like protein antibody
    • CSE 1L antibody
    • CSE1 antibody
    • CSE1 chromosome segregation 1 like (yeast) antibody
    • CSE1 chromosome segregation 1 like antibody
    • CSE1 chromosome segregation 1 like protein antibody
    • CSE1L antibody
    • Exp 2 antibody
    • Exp2 antibody
    • Exportin 2 antibody
    • Exportin-2 antibody
    • Exportin2 antibody
    • Importin alpha re exporter antibody
    • Importin-alpha re-exporter antibody
    • MGC117283 antibody
    • MGC130036 antibody
    • MGC130037 antibody
    • XPO 2 antibody
    • XPO2 antibody
    • XPO2_HUMAN antibody
    see all

Anti-Cellular Apoptosis Susceptibility antibody 画像

  • Cellular Apoptosis Susceptibility antibody (ab54674) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

  • Predicted band size : 110 kDa
    Cellular Apoptosis Susceptibility antibody (ab54674) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • ab54674 at 10 µg/ml staining Cellular Apoptosis Susceptibility human Hela cells by Immunocytochemistry / Immunofluorescence.

  • All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab54674) at 1/2500 dilution

    Lane 1 : Hela extract 25 ug
    Lane 2 : RPE-1 extract 25 ug
    Lane 3 : Hela extract 50 ug
    Lane 4 : RPE-1 extract 25 ug

    Donkey LICOR anti-mouse IRDye680 at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa (possible cross reactivity),66 kDa (possible cross reactivity).

    Exposure time : 3 minutes

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab54674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54674, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cellular Apoptosis Susceptibility antibody (ab54674) 使用論文

This product has been referenced in:
  • Winkler J  et al. Prosurvival function of the cellular apoptosis susceptibility/importin-a1 transport cycle is repressed by p53 in liver cancer. Hepatology 60:884-95 (2014). Read more (PubMed: 24799195) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount 25 µg
Specification HeLa and RPE-1
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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投稿 Oct 10 2012