製品の概要

  • 製品名Anti-Cellubrevin antibody
    Cellubrevin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cellubrevin
  • アプリケーション適用あり: WB, ICC/IF, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Cellubrevin.

    (Peptide available as ab43579.)

  • ポジティブ・コントロール
    • ab43080 gave a positive signal in the following Tissue Lysates: Mouse Brain (Data not shown), Mouse Testis, Rat Brain and Rat Testis. It also gave a positive result in PC12 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 22936810
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
  • 配列類似性Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CEB antibody
    • Cellubrevin antibody
    • Synaptobrevin 3 antibody
    • Synaptobrevin-3 antibody
    • VAMP 3 antibody
    • VAMP-3 antibody
    • VAMP3 antibody
    • VAMP3_HUMAN antibody
    • Vesicle associated membrane protein 3 antibody
    • Vesicle-associated membrane protein 3 antibody
    see all

Anti-Cellubrevin antibody 画像

  • All lanes : Anti-Cellubrevin antibody (ab43080) at 1 µg/ml

    Lane 1 : Testis (Mouse) Mouse Tissue Lysate
    Lane 2 : Brain (Rat) Rat Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab43080 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Cellubrevin staining in Mouse normal testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cellubrevin antibody (ab43080) 使用論文

This product has been referenced in:
  • Schwenk RW  et al. Overexpression of Vesicle-associated Membrane Protein (VAMP) 3, but Not VAMP2, Protects Glucose Transporter (GLUT) 4 Protein Translocation in an in Vitro Model of Cardiac Insulin Resistance. J Biol Chem 287:37530-9 (2012). WB, IP ; Rat . Read more (PubMed: 22936810) »
  • Mendez M  et al. Vesicle-associated Membrane Protein-2 (VAMP2) Mediates cAMP-stimulated Renin Release in Mouse Juxtaglomerular Cells. J Biol Chem 286:28608-18 (2011). WB ; Mouse . Read more (PubMed: 21708949) »

See all 2 Publications for this product

Product Wall


I have run a BLAST for the immunogen of VAMP3 and according to this it is specific to this protein. Experimentally I cannot provide further details as we have not tested it in house.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (Oligodendrocytes)
Total protein in input 50 µg
Specification Oligodendrocytes
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Feb 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Fibroblast)
Specification Fibroblast
Fixative Formaldehyde
Permeabilization Yes - 0.03% Trition
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Astrocytes)
Loading amount 30 µg
Specification Astrocytes
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 25 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (brain sections)
Specification brain sections
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"