Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)

製品の概要

  • 製品名Cell Viability Assay Kit (Fluorometric - Near InfraRed)
    Cell Viability キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 製品の概要

    Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. The proprietary far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.

    ab112123 is an effective tool of labeling cells for the investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112123 is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. It comes with reagents sufficient to perform 200 assays in a 96-well plate or 800 assays in a 384-well plate. The kit provides all the essential components with an optimized protocol for microplate reader analysis of cell viability.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112123 should be stored desiccated.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

アプリケーション

Our Abpromise guarantee covers the use of ab112123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

Cell Viability Assay Kit (Fluorometric - Near InfraRed) 画像

  • CHO-K1 cell number response measured with ab112123.
    CHO-K1 cells at 0 to 50,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Near InfraRed Dye solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 635/670 nm.

プロトコール

Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123) 使用論文

ab112123 has not yet been referenced specifically in any publications.

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Le filtre dufluorophore correspondant du kit ab112123 est le filtre pourCy5. Lesfiltres pour leCy3 et le Texas Red ne sont donc pas appropriés pour l'utilisation du kit ab112123.

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MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"