Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Cell Viability Assay Kit (Fluorometric - Dual Green/Red)
Cell Viability キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. ab112121 uses two non-fluorescent indicators: The CellGreen fluorescent dye for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic CellGreen fluorescent dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells. Cells grown in black-wall plates can be stained and quantified in less than two hours.
ab112121 is more robust and accurate than the other viability assays. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, fluorescence microscope, and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for both proliferating and non-proliferating cells (either suspension or adherent cells). ab112121 comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).
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特記事項
ab112121 should be stored desiccated.Assay kit comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
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試験プラットフォーム
Microplate reader, Fluor. microscope, Flow cyt.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 2 x 96 tests Assay Buffer 1 x 20ml CellGreen fluorescent dye 2 vials DMSO 1 x 100µl Propidium Iodide 1 x 40µl -
研究分野
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関連性
Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions. -
別名
- cell tracking
画像
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CHO-K1 cell number response was measured with ab112121. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab112121 は論文での使用が確認できていません。