製品の概要

  • 製品名Cell Cytotoxicity Assay Kit (Fluorometric)
    Cell Cytotoxicity キット 製品一覧
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 種交差性
    交差種: Human, Chinese Hamster
    交差が予測される動物種: all Mammals
  • 製品の概要

    The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability.
    ab112119 Cell Cytotoxicity Assay Kit provides a fast, simple, accurate and homogeneous assay for the fluorimetric detection of viable cells. ab112119 is based on the observation that oxidized non-fluorescent blue resazurin is reduced to a red fluorescent dye (resorufin) by accepting an electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.

    ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112119 should be stored desiccated

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112119 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Cell Cytotoxicity Assay Kit (Fluorometric) 画像

    • CHO-K1 cell number response was measured with ab112119. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm. The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit from the blank controls was 60 cells/well (n=6).

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    Cell Cytotoxicity Assay Kit (Fluorometric) (ab112119) 使用論文

    ab112119 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab112119.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"