Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)

製品の概要

  • 製品名Cell Cytotoxicity Assay Kit (Colorimetric)
    Cell Cytotoxicity キット 製品一覧
  • テスト
    1 x 1000 test
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 種交差性
    交差種: Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.

    ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
    ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112118 should be stored desiccated.

    As low as 300 cells can be accurately quantified using ab112118.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112118 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Cell Cytotoxicity Assay Kit (Colorimetric) 画像

    • •HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.

      •NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.

    • CHO-K1 cell number response was measured with ab112118. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 oC. The absorbance intensity was measured at 570 nm and 605 nm. The ratio of OD570/OD605 is proportional to the number of cells as indicated.

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    Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118) 使用論文

    ab112118 has not yet been referenced specifically in any publications.

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    ab112118 is compatible with media containing phenol red. Any potential background will be subtracted in your background wells. DTT should not be present as it interferes with the assay. For the background control wells (media without cells), pleas...

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    Increased concentrations of dopamine reduce cell survival independently of α-synuclein presence.
    Normal SH-SY5Y cells and SH-SY5Y cells transfected with 1 µg α-synuclein siRNA were treated with varying concentrations of dopamine with and without MG-132 proteasome inhibitor for 72 h. Cell viability was measured using ab112118 cell cytotoxicity assay kit (abcam, Cambridge, UK). Data was analysed using one-way ANOVA corrected by post-hoc Sheffé's test (* p < 0.05, ** p < 0.01) and expressed as a relative percentage of survival compared to PBS vehicle control. Error bars represent ± standard deviation of four independent replicates.
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    投稿 Sep 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"