Rabbit polyclonal to Cell adhesion molecule 4
Mouse, Rat, Human
Synthetic peptide derived from an internal region of human Cell adhesion molecule 4.
A549 cells, extracts from RAW264.7 cells, human brain tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21482674
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
1/50 - 1/100.
1/500 - 1/1000.
Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.
Expressed in brain, prostate, brain, kidney and some other organs.
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Information by UniProt
CADM 4 antibody
Western blot - Cell adhesion molecule 4 antibody (ab69605)
All lanes : Anti-Cell adhesion molecule 4 antibody (ab69605) at 1/500 dilution Lane 1 : extracts from RAW264.7 cells Lane 2 : extracts from RAW264.7 cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 43 kDa Observed band size : 43 kDa Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Cell adhesion molecule 4 antibody (ab69605)
A549 cells,stained with ab69605 at 1/500-1/1000 dilution, with and without the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cell adhesion molecule 4 antibody (ab69605)
Human brain tissue stained with ab69605 at 1/50-1/100 dilution, with and without the immunising peptide.
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