製品の概要

  • 製品名Anti-CELF3 antibody
    CELF3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CELF3
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 268-401 of Human CELF3 (Q5SZQ8).

  • ポジティブ・コントロール
    • RT-4 cell lysate; Human cerebral cortex tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

ターゲット情報

  • 機能RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.
  • 組織特異性Expressed in brain.
  • 配列類似性Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bruno-like protein 1 antibody
    • BRUNOL1 antibody
    • CAG repeat protein 4 antibody
    • CAGH4 antibody
    • CELF-3 antibody
    • CELF3 antibody
    • CELF3_HUMAN antibody
    • CUG-BP- and ETR-3-like factor 3 antibody
    • CUGBP Elav-like family member 3 antibody
    • ELAV-type RNA-binding protein 1 antibody
    • ERDA4 antibody
    • ETR-1 antibody
    • Expanded repeat domain protein CAG/CTG 4 antibody
    • RNA-binding protein BRUNOL-1 antibody
    • TNRC4 antibody
    • Trinucleotide repeat-containing gene 4 protein antibody
    see all

Anti-CELF3 antibody 画像

  • All lanes : Anti-CELF3 antibody (ab121249) at 1/250 dilution

    Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Liver lysate
    Lane 6 : Tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human cerebral cortex, using 1/35 ab121249, shows distinct cytoplasmic positivity in neurons.

Anti-CELF3 antibody (ab121249) 使用論文

ab121249 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human brain motor cortex)
Antigen retrieval step Heat mediated
Specification Human brain motor cortex
Blocking step Protein Block Novocastra from Leica Biosystems (# RE7102-CE) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formalin
Username

Guinevere Lourenco

Verified customer

投稿 Nov 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"