製品の概要

  • 製品名
    Anti-CEE antibody
  • 製品の詳細
    Rabbit polyclonal to CEE
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 277-327 of Human CEE (NP_057033.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
    • 配列類似性
      Belongs to the GET4 family.
    • 細胞内局在
      Cytoplasm > cytosol.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C7orf20 antibody
      • CGI 20 antibody
      • CGI20 antibody
      • Conserved edge expressed protein antibody
      • Conserved edge protein antibody
      • Conserved edge-expressed protein antibody
      • GET 4 antibody
      • GET4 antibody
      • GET4_HUMAN antibody
      • Golgi to ER traffic protein 4 homolog (S. cerevisiae) antibody
      • Golgi to ER traffic protein 4 homolog antibody
      • Transmembrane domain recognition complex 35 kDa subunit antibody
      • Transmembrane domain recognition complex 35kDa antibody
      • TRC35 antibody
      • UPF0363 protein C7orf20 antibody
      see all

    画像

    • All lanes : Anti-CEE antibody (ab93801) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 37 kDa


      Exposure time : 3 minutes
    • Detection of CEE in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93801 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

    参考文献

    ab93801 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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