製品の概要

  • 製品名Anti-CEBP gamma antibody
    CEBP gamma 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CEBP gamma
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-151 of Human CEBP gamma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites.
  • 配列類似性Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C/EBP gamma antibody
    • CCAAT/enhancer binding protein (C/EBP) gamma antibody
    • CCAAT/enhancer binding protein gamma antibody
    • CCAAT/enhancer-binding protein gamma antibody
    • CEBPG antibody
    • CEBPG_HUMAN antibody
    • GPE1BP antibody
    • IG/EBP 1 antibody
    • IG/EBP1 antibody
    see all

Anti-CEBP gamma antibody 画像

  • All lanes : Anti-CEBP gamma antibody (ab54899) at 1/1000 dilution

    Lane 1 : Mouse adipocyte lysate - untreated
    Lane 2 : Mouse adipocyte lysate - 5nm rapamycin treatment for 48 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Kun Dong

    See Abreview

  • Western blot against tagged recombinant protein immunogen using ab54899 CEBP gamma antibody at 1ug/ml. Predicted band size of immunogen is 43 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Anti-CEBP gamma antibody (ab54899) 使用論文

ab54899 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (12.5%)
Sample Mouse Cell lysate - other (adipocyte)
Specification adipocyte
Treatment 5nm rapamycin for 48 hours
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. KUN DONG

Verified customer

投稿 Jan 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"