製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CEBP Delta
  • 特異性
    ab65081 detects endogenous levels of total CEBP Delta protein.
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human CEBP Delta (C terminal).

  • ポジティブ・コントロール
    • Extracts from LOVO cells and RAW264.7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.

Peptide ELISA tested only.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.
  • 配列類似性
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C/EBP delta antibody
    • C/EBP related protein 3 antibody
    • CCAAT/enhancer binding protein (C/EBP) delta antibody
    • CCAAT/enhancer binding protein delta antibody
    • CCAAT/enhancer-binding protein delta antibody
    • CEBP D antibody
    • CEBPD antibody
    • CEBPD_HUMAN antibody
    • CELF antibody
    • Crp 3 antibody
    • Crp3 antibody
    • NF IL6 beta antibody
    • NF-IL6-beta antibody
    • Nuclear factor NF IL6 beta antibody
    • Nuclear factor NF-IL6-beta antibody
    see all

画像

  • Immunofluorescence analysis of murine spinal cord cells, staining CEBP Delta with ab65081 .

    Cells were fixed with acetone and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/50 in 1% goat serum) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/250) ab150073) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CEBP Delta antibody (ab65081) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from RAW264.7 cells
    Lane 3 : Extracts from RAW264.7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

参考文献

This product has been referenced in:
  • Wang YH  et al. CEBPD amplification and overexpression in urothelial carcinoma: a driver of tumor metastasis indicating adverse prognosis. Oncotarget 6:31069-84 (2015). IHC . Read more (PubMed: 26307680) »
  • Utheim O  et al. Serum-free and xenobiotic-free preservation of cultured human limbal epithelial cells. PLoS One 10:e0118517 (2015). Read more (PubMed: 25734654) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spinal cord)
Specification
Spinal cord
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 21 2012

DISCOUNT CODE: XXXXXXXXXXX
Expiration date: 12-01-2013

I am very pleased to hear you would like to accept our offer and test ab65081 in IHC-P / IHC-Fr. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080)
Loading amount
100000 cells
Specification
HT1080
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hepatocellular carcinoma)
Loading amount
40 µg
Specification
Hepatocellular carcinoma
Treatment
5 mM/L of acetaldehyde for 24 Hours
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ratnakar Singh

Verified customer

投稿 Apr 02 2012

Thank you for contacting us. As you say, the Anti-CEBP Delta antibody (ab65081) has not as yet been assessed in IHC. However, unfortunately, due to the size of our catalogue we are unable to provide free samples to researchers due to the logistics invo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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