製品の概要

  • 製品名Anti-CEBP Beta (phospho T235 + T188) antibody
    CEBP Beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CEBP Beta (phospho T235 + T188)
  • 特異性This antibody detects endogenous levels of CEBP Beta only when phosphorylated at threonine 235/188.
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human CEBP Beta around the phosphorylation site of threonine 235/188.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB Use at an assay dependent concentration. PubMed: 22144112

ターゲット情報

  • 機能Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • 組織特異性Expressed at low levels in the lung, kidney and spleen.
  • 配列類似性Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all

Anti-CEBP Beta (phospho T235 + T188) antibody 画像

  • ab52194 staining CEBP Beta (phospho T335 + T188) in Human MDA-MB-468 breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde + 2% sucrose and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (in PBS + 1% BSA) for 30 minutes at 37°C.

    From left - DAPI (blue), MUC1-C (green), CERP Beta phospho T335 + T188 (red), Merge, Zoom.

    Top - CshRNA, Bottom - MUC1shRNA.

    Magnification - x63, Zoom (from pink boxes) - x252.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab52194 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52194 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

Anti-CEBP Beta (phospho T235 + T188) antibody (ab52194) 使用論文

This product has been referenced in:
  • Alam M  et al. MUC1-C Oncoprotein Activates ERK->C/EBPß Signaling and Induction of Aldehyde Dehydrogenase 1A1 in Breast Cancer Cells. J Biol Chem 288:30892-903 (2013). ICC/IF ; Human . Read more (PubMed: 24043631) »
  • Chen CJ  et al. Glutamate released by Japanese encephalitis virus-infected microglia involves TNF-a signaling and contributes to neuronal death. Glia 60:487-501 (2012). WB . Read more (PubMed: 22144112) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab52194.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"