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RabMAb

Anti-CEBP Beta 抗体 [E299] (ab32358)

製品の概要

  • 製品名
    Anti-CEBP Beta antibody [E299]
    CEBP Beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E299] to CEBP Beta
  • 特異性
    This antibody is specific for the three CEBPB isoforms (LAP*, LAP and LIP). According to BLAST analysis, the antibody could cross-react with CEBP epsilon (32, 27 and 14kDa, 82% homology) and CEBP alpha (42kDa, 30kDa, 73% homology) in human, mouse and rat. Please be aware that this has not been confirmed experimentally. However, this could explain the background that could possibly be obtained in WB with this antibody. Please contact our Scientific Support if you have any questions.
  • アプリケーション
    適用あり: EMSA, WB, ICC/IF, Flow Cyt, IPmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C terminus of Rat CEBPB.

  • ポジティブ・コントロール
    • WB: PC12 cell lysate. IF: Hela cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
EMSA Use at an assay dependent concentration. PubMed: 18462018
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
    • 組織特異性
      Expressed at low levels in the lung, kidney and spleen.
    • 配列類似性
      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • ドメイン
      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • 翻訳後修飾
      Sumoylated by polymeric chains of SUMO2 or SUMO3.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AGP/EBP antibody
      • C EBP beta antibody
      • C/EBP beta antibody
      • C/EBP related protein 2 antibody
      • CCAAT Enhancer Binding Protein beta antibody
      • CCAAT/enhancer-binding protein beta antibody
      • CEBPB antibody
      • CEBPB_HUMAN antibody
      • CRP2 antibody
      • IL 6DBP antibody
      • IL6DBP antibody
      • Interleukin 6 dependent binding protein antibody
      • LAP antibody
      • Liver activator protein antibody
      • Liver enriched transcriptional activator antibody
      • NF IL6 antibody
      • NFIL6 antibody
      • Nuclear factor NF IL6 antibody
      • Nuclear factor NF-IL6 antibody
      • SF B antibody
      • SFB antibody
      • Silencer factor B antibody
      • TCF-5 antibody
      • TCF5 antibody
      • Transcription factor 5 antibody
      see all

    画像

    • Anti-CEBP Beta antibody [E299] (ab32358) at 1/1000 dilution + PC12 cell lysate.

      Predicted band size : 36 kDa
      Observed bands
      LAP*: 38kDa
      LAP: 35kDa
      LIP: 20kDa
    • Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.
    • Overlay histogram showing HeLa cells stained with ab32358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32358, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-CEBP Beta antibody [E299] (ab32358) at 1/500 dilution

      Lane 1 : 3T3 Cells
      Lane 2 : MCF7 Cells
      Lane 3 : PC12 Cells
      Lane 4 : Rat Spleen Lysate
      Lane 5 : Rat Kidney Lysate
      Lane 6 : Rat Heart Lysate
      Lane 7 : Rat Brain Lysate
      Lane 8 : Mouse Spleen Lysate
      Lane 9 : Mouse Kidney Lysate
      Lane 10 : Mouse Heart Lysate
      Lane 11 : Mouse Brain Lysate


      Predicted band size : 36 kDa

    参考文献

    This product has been referenced in:
    • Pulido-Salgado M  et al. Myeloid C/EBPß deficiency reshapes microglial gene expression and is protective in experimental autoimmune encephalomyelitis. J Neuroinflammation 14:54 (2017). Read more (PubMed: 28302135) »
    • Pyle CJ  et al. Zinc Modulates Endotoxin-Induced Human Macrophage Inflammation through ZIP8 Induction and C/EBPß Inhibition. PLoS One 12:e0169531 (2017). Read more (PubMed: 28056086) »

    See all 16 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (C3H10T cells)
    Specification
    C3H10T cells
    Treatment
    25ng/ml (BMP4)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 26 2015

    Vielen Dank für Ihre Email.

    Ich habe mich an das Labor gewandt und habe nun eine Rückmeldung erhalten. Der Antikörper wurde im Labor für IHC und FC getestet und die erhaltenen Ergebnisse waren negativ. Das Labor schlie&szli...

    Read More

    Thank you for your inquiry. I am happy to confirm that when you are submitting the Abreview, you can choose to show your name and other scientists will also be able to contact you if you choose to permit this.

    DISCOUNT CODE: xxxxxx

    E...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (mcf7)
    Loading amount
    100000 cells
    Specification
    mcf7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 May 10 2012

    Thank you for contacting us.

    Yes, that would be lovely. As always, we would need the customers email and the institution he/she is working for.
    Looking forward to hearing from you.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (HT1080 Cell line)
    Loading amount
    100000 cells
    Specification
    HT1080 Cell line
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Neil Taylor

    Verified customer

    投稿 Feb 08 2010

    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
    Specification
    Mouse Embryonic Fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Username

    Dr. Neil Taylor

    Verified customer

    投稿 Oct 06 2009

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HT1080 Cell line)
    Specification
    HT1080 Cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Username

    Dr. Neil Taylor

    Verified customer

    投稿 Oct 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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