Anti-CEBP Beta 抗体 [18F8] (ab15049)
Key features and details
- Mouse monoclonal [18F8] to CEBP Beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CEBP Beta antibody [18F8]
CEBP Beta 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [18F8] to CEBP Beta -
由来種
Mouse -
特異性
Ab15049 only detects the LAP* isoform of CEBPG beta. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse CEBP Beta aa 1-19 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:MHRLLAWDAACLPPPPAAF
(Peptide available asab39810) -
ポジティブ・コントロール
- Transfected 293T cells, P388D1 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
18F8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15049の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810).
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).Can be blocked with Mouse CEBP Beta peptide (ab39810). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
組織特異性
Expressed at low levels in the lung, kidney and spleen. -
配列類似性
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻訳後修飾
Sumoylated by polymeric chains of SUMO2 or SUMO3. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 12608 Mouse
- SwissProt: P28033 Mouse
- Unigene: 439656 Mouse
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別名
- AGP/EBP antibody
- C EBP beta antibody
- C/EBP beta antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (8)
ab15049 は 8 報の論文で使用されています。
- Fafián-Labora JA et al. Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage. Cell Metab 32:71-86.e5 (2020). PubMed: 32574561
- Liu SP et al. Secukinumab attenuates reactive astrogliosis via IL-17RA/(C/EBPß)/SIRT1 pathway in a rat model of germinal matrix hemorrhage. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31020769
- Romanel A et al. Inherited determinants of early recurrent somatic mutations in prostate cancer. Nat Commun 8:48 (2017). PubMed: 28663546
- Jang MA et al. a-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS One 12:e0170699 (2017). PubMed: 28114367
- Liu Q et al. Cyclin D1 and C/EBPß LAP1 operate in a common pathway to promote mammary epithelial cell differentiation. Mol Cell Biol 34:3168-79 (2014). PubMed: 24912680
- Atwood AA et al. Negative Regulation of C/EBPbeta1 by Sumoylation in Breast Cancer Cells. PLoS One 6:e25205 (2011). WB ; Human . PubMed: 21980398
- Atwood AA & Sealy L Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence. Oncogene 29:6004-15 (2010). WB ; Human . PubMed: 20818427
- Su WC et al. Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene. J Biol Chem 278:51150-8 (2003). PubMed: 14530280