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RabMAb

Anti-CEBP Alpha 抗体 [EP709Y] (ab40764)

製品の概要

  • 製品名
    Anti-CEBP Alpha antibody [EP709Y]
    CEBP Alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP709Y] to CEBP Alpha
  • アプリケーション
    適用あり: EMSA, WB, Flow Cyt, IPmore details
    適用なし: ICC/IF or IHC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the C-term of human CEBP Alpha

  • ポジティブ・コントロール
    • U937 cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
EMSA Use at an assay dependent concentration. PubMed: 22184113
WB 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF or IHC.
  • ターゲット情報

    • 機能
      C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • 配列類似性
      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Anti-CEBP Alpha antibody [EP709Y] 画像

    • Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg
      Developed using the ECL technique

      Performed under non-reducing conditions.

      Predicted band size : 43 kDa
      Observed band size : 43 kDa


      Exposure time : 12 seconds
    • Overlay histogram showing HeLa cells stained with ab40764 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40764, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/1000 dilution + Human ESC (HUES7) whole cell lysate at 10 µg with 5% milk for 30 minutes at RT.

      Secondary
      Monoclonal Donkey Anti-rabbit IgG HRP at 1/10000 dilution

      Predicted band size : 43 kDa

      This image is courtesy of an anonymous abreview.

      See Abreview

    Anti-CEBP Alpha antibody [EP709Y] (ab40764) 使用論文

    This product has been referenced in:
    • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »
    • Schwind L  et al. Inhibition of Protein Kinase CK2 Prevents Adipogenic Differentiation of Mesenchymal Stem Cells Like C3H/10T1/2 Cells. Pharmaceuticals (Basel) 10:N/A (2017). Read more (PubMed: 28208768) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (fat)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    20 µg
    Specification
    fat
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ESC (HUES7))
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    10 µg
    Specification
    ESC (HUES7)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 09 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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